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CRIMIC CR02798

Gene mAChR-A

CG4356

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2R:24381985..24381986	60C7	FBti0228149	97616

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGATGAATTCGCGATGTGTCGC	ACTCATTTCCTGCAGCTGCGAGAT

Images

Construct Design Sequences

sgRNA sequence	CAACAGTTCGGGGCCAACTCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGATACGTATCTCTTTGACCGCTGGACTTGTTTCAATTTATGTGTGCCATTGGCAATCGGCTGGGTAATGGATATATGCTGCGATTTCCGGCCACTTGTTTTCATTCCAAATGCACTGCCCACATGCATGATGTGTTTCTGTGTCCTGTGTGTCTGAGTGTGCCACATTTGGTTGAGCAGGGGCACAGTTGCATCCAGAG	TTGGCCCCGAACTGTTGTTCTTGTTCTAGTTTGCCTGTCAGTGTCGTTGGAGTGGTCCACTCTAATGCGTGTTTGTTGAAATGCTAAACCGATGCCACATCCTGTCTTCTCTCTTTCTTCACATCGCAGATGCACCTCGATGAACGTGATGCGGGACAACTACTCGATGGGCGGCAGCGTGAGCGGCGTGCGTCCGCCCA

 

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