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CRIMIC CR02799

Gene Sox100B

CG15552

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	3R:31072339..31072340	100B2	FBti0228150	97617

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTAATCAATCCATGGGTCTGCTG	GCCTGTTGCTGTCGAATTCCAT

Images

Construct Design Sequences

sgRNA sequence	AGAGATAGGCGTTCCAGGGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCTAACATTTTACCGGCCAGGCACAGGATGCAGTCAGGCATATCGAATAAACTTGACACACGCACACCCAACTCAATTGAAGGATTTACCTAACAAATCCTTGCCTTGATCATGTTTACTTAGGATAAATAATTCAATATACTCCGAAAAGGTGGGCAGCCAATGGGAAATCTGGGGCACTCTGCCATTGTGTTCCGCCC	CTGGAACGCCTATCTCTTTGATTGAATCTCTGTCTTCCTCGACGCTCGACATGGCTAAATAAAAATGAATGAATAGATAAGAACAATCTGGGTGCTCGGATATGTGTGTTAGAGTTCGATTTATAGTTAGTGAGGGGTGTCCTAATATATATCACTGCCGAATATATGTAGAAAAAGGCTTTTACATTTTTCTATAGAAG

 

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