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CRIMIC CR02807

Gene bwa

CG13969

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:20068136..20068137	38B2-38B3	FBti0228153	97620

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTCTCAAAGTGAACTCAAGTCCA	TATCACATCTAAAGGGTGCATGGA

Images

Construct Design Sequences

sgRNA sequence	AATGCGTTGTGCACTTCAATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATAGTATGTCGTAATTTACAATCTTGCGTTTCAAATAGAACGCAGTATCATCAGTTCGGTCAATGTAAACTGGGGAGCCTTAAGTTACTTTGTAGAACGGCCTTTCATATGGTGCAGATACACTTCCGCTGGCTAACGATAATGGGAATGGCTTTTAATTGATTTGGTCGACATGAGTAATCGTTCGCCGTGGCCCATT	GAAGTGCACAACGCATTTCGGCTTTGTTTCAACTGAGTTGTTCGCCCAGCAAACGGAGTGAATAACCCCGTTCTTGACACTCTGCTGCACAATAACCAGCGGCACTTGTGTTGATGAAATCGTTAATATCTCTATAGTGGGAAGTGTGAATAATTGCGGCATATCCCAGTTTCTTATCACTCACTGGTTTTAATAGTATA

 

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