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CRIMIC CR02811

Gene Pp2A-29B

CG17291

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:8366489..8366490	29B2	FBti0228156	97623

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTTGCCTGCAGAATAGAAGACC	CTTATACTTATACATACATAAATGAAGGAT

Images

Construct Design Sequences

sgRNA sequence	CGCAGATAAAGCAGGGAGTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAGCAGAAACAGCAGAAAAAATGGCAGCAAGCGACAAATCGGTCGACGATTCACTATATCCCATTGCGGTTCTAATCGATGAACTGAAAAACGAGGACGTTCAGGTTTGTCATGTCCTAAAACCTGAAATCCAATTGTATCTCGCGAAGAATGTGCAGAAATATTCTAGTGATTAAATTTTGGGGTAGCGTGCCCACAC	TCCCTGCTTTATCTGCGATTGTATTTGTGCAAAAATGCGTCACGGTGTGTGTGTGCGTGTGTGCATGGGCATTTGTGTTGGTGCGAGTGTGTGCATCGAAATTATACCGAATTTCGATATACACAAGATTAGCGTTTTGCGAAAAAAATTACTATTATCAATTAATTGCAATTATGGGTATACGTCTTTATCCGCTTTTT

 

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