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CRIMIC CR02816

Gene dbr

CG11371

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:67817..67818	21B1	FBti0228158	97625

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTGGGCTTGACGCTTTGAGATAGG	TTCCCTCGCATTCTTAGGCTGGAA

Images

Construct Design Sequences

sgRNA sequence	TTTGATACTTATTCAGATCTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGTAACATGGATGCTCAGTTTGAGCACTTGTGCCGCATTTGCGCTGCCAACACGAAAAGCAAGACGAATTCCGTGGTTGAAAGTGTGTTTATTTTTAAAACACAGGGACTGAAGGATAAAATATCTCGACACCTTTATCTAAATGTAAGTAAATAAAAAGGAACAGCCTGTTTGGCGCTGCATCAAATTTAATCCTAGA	TCTGAATAAGTATCAAAACAAGACCTCCGCGTACTTGCTAAGACTCGACATTTAAAAAACGTATTTCTTTTTAGGTAGCTGAAGAAGATCCTCTGCCAAAAGTGCTCTGCAAATCTTGTTACCGCCAAGTAGAGGCCACGGCGTCGCTTTCAAATATAGCAAAACATACTCAGCTCGTGTTTCGGGACTTTCTCCTTAGT

 

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