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CRIMIC CR02817

Gene Rcd6

CG11175

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:20630603..20630604	57A8	FBti0228159	97626

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGCCAGTGAAAAGCAGTCGT	TCGATGGCAGCCAGAGATATAGAT

Images

Construct Design Sequences

sgRNA sequence	AACGGTAACGGACAGTCCGACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAGAAGCGGCCGCCGGGCAACGGCAATAGCATACAAAACTTTTGGAACGATCGCATTATGGAGCGCTTCATAAAGGCGAATCTATTCATTATCTGCTGCGTGGTGGCGGTTCTATTGCTGGTAAGTATATCTCTAGGTGAGATATATATCTCCATATCGCCACCATCTCTCCAACCCACTATATCTTCGTCCGTCCGTCG	GACTGTCCGTTACCGTTATCAGCGTGAAAACATTTCTGCTCCATTAAGTGAAAATGAAATGAAATCGTTTTTGCGCAAATGTAAAAATTTCATCTCTTTTTGTTGCTGTCCAAGTTGACAGCTGTGGAGGGAGGAGAGGTGCGAGGTAGCTCATTAATTGATTGATGGATGGTTGCGGAGCACAGTTGTCTCCGGCTTTT

 

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