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CRIMIC CR02822

Gene CG34221

CG34221

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:10198589..10198590	46F5	FBti0228163	97630

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTCCCAAATTGAGCCATCAGTAGG	GGACTTACTTCAGCTTGTCGCTTG

Images

Construct Design Sequences

sgRNA sequence	ATAATAACCCCAGGACGATGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGACAATTTACCACCCAGTGAGTAGCGGCCAAATGCTCTTCTATGTATTTCCGTCTGGCCCAAATTGGAGTTGACCGGGCCAAAAGTGTGCTTCCTGTGCGGCTGACAGCTTCTGTTACGCCCACATCAGAATCTATCTGCAGATGAAGGCGGTCCCATCATCGTTGGCTGGCCTGTCAATAATAATAACCCCAGGACG	ATGAGGTCCTCGGATGCGTGTTATTATGCTAATTGTATTGTTCGGCTGTTTATGAGCTGTTTTGGGGACAGAGGGGGGGGGGCGATAACCTCTTCCTTACGTTGGCATACGAATCGTAATCTTTATACTTGTTTCCCTTGCAGGCATTAAGCTTTCTGTTCATCACTTGCATGATGTTCGCACTGGCAGGCGGCCATCCA

 

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