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CRIMIC CR02823

Gene Dcr-2

CG6493

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:17574979..17581525	54C10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CTCGATGATCCAGAACAATCCAC	CTCGATGATCCAGAACAATCCAC

Images

Construct Design Sequences

sgRNA sequence	ATCGCAATTGCAGTGTGTAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGCCCGACAACAGGCGATGGCCGTCAGGCGGTGCACCAACTTTAAGGTTGGCTTTTATGTGGGTGAACAGGGAGTGGATGACTGGACGCGCGGAATGTGGAGTGATGAAATCAAAAAAAACCAAGTGAGTATAGGATGGCGGAGGCATTTTTTATATACGCATATGATCACGTACGGACTAAACACTACACTACCACTA	CACACTGCAATTGCGATCACCACTTGAGTCGAAAAATTTGGGATCAGCATTTTTATGGGCTCGTAAGGGGTCGTGTTTTGCTAAGCCATCACAAAATCATCATTTCGTCAATTGATGTATTTTATTTGTTTTTTTTTTTTTTTGTAGTATGTTTATTTGTTTATTTATTTATTTTATTTGTTTATTTAAAATTAACTTGA

 

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