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CRIMIC CR02825

Gene CG13428

CG13428

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:20540100..20540101	57A5	FBti0228164	97631

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTGACCCTCTTCGGAGTCGAT	CAATGTATCACGACGGGACTGGAA

Images

Construct Design Sequences

sgRNA sequence	CCCAGGAGGCAGTGCACTGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTCGGTGGTTTAGTGACCTATCAACGGGCTTAATCGTCTCTCCTTGCAGGAACCAAAGTTCGTCGGTTTGTTCAGGCGGGTGAGTACACAAAGAATGGGTCAAACTGAAGGCCAGTGATTGCTAAGACTATTGGCCCTACAAACGAAACTAAAGGCGCCAGAACAGATGAAGCAACTAGATGCCCAGGAGGCAGTGCAC	TGGCGGCGCATTCACAATTTAGCACTCCTAATGCAAAATTATGTTAAATGCCTACTTCTAGGCAAGGCGCCATGTCCCCGCAATGAAAACCGCCCTTCTTCACGGCAACGAACCGCCTCCACCTCCGAAATTCCGAGTAAGAACTTAGTAAAATGTAGAAAACTAGAACATTTATATAAGCAATTGTGTTGCGGCCGTAT

 

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