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CRIMIC CR02831

Gene didum

CG2146

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:7500793..7500794	43C7-43D1	FBti0228167	97634

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGGTGTCGTCTGCAAATGGT	CCGGAGTCCGTACATATCTTCAAG

Images

Construct Design Sequences

sgRNA sequence	GTGTCTTATCGACAGGGGAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAATCGCACCCATAACGTGATTTCCCCCGTCCGAAGTGTGTGTGTGTGTGTGTGTGTGTGTGCAGGCCATTCGACAGTTCTTGGCCCTGATTTGTTCCCATGGCAGGTGATTAGCCATTGATGACTGCATCGCAGCACCCGAAATGTTTAAAAATGATTCATCTACATACAATATTCGCGGGTGTCTTATCGACAGGG	GAATGGGTTGTGTGATAAGCTCGATTGATAAGACACCAGGTCGGGTGACGTCTCTAGAGGGGATCGGGCATAACTCCCATTCATAAAACTTGTTAATTCGGCTCACTTCTTTCGCAGGGCGCCAAGATCTGGGTGCCCCATGCGGATCTGGTGTGGGAGAGCGCCACCTTGGAGGAGAGCTACCGCAAGGGCGCCGGCTT

 

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