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CRIMIC CR02833

Gene SdhA

CG17246

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:19395610..19395611	56D3	FBti0228168	97635

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCAAACACCCCATCTTACAAGC	TGGTGCACACCTAGCTTTCCACTA

Images

Construct Design Sequences

sgRNA sequence	TTAAGAGTGGTTATACAACCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTAAAACCTCGTTTTCGGTCCAATCTCTTGCAGAATCTTGCAGTAGAACCTCACAGCCCAGCCAAACATGTCCGGAATCATGCGTGTGCCATCGATTTTGGCCAAAAATGCAGTCGCCTCCATGGTAAGTAGTCCCCATTTTGGAAGTGAAAACCCAGGCTCCTCTGTGGCAAACGAAATGATTTAAGAGTGGTTATACA	ACCGGGTCTACACAGAACCCAGCCAGCCAGCTCGTGATGTGACAACAGAAAAAGACCAACGAATTGGCCAAAAACGCGCCACCCGTACTTGTGTTATGTAATTTGTGAGCAAATTGTGGGACACAGGTGCATTAAATTATTCCCTTTGCTAGATTATATTAATTTTCATATAGCGAATATCATTCCAAGCAAGTGTTCAG

 

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