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CRIMIC CR02834

Gene ZnT77C

CG5130

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:20486704..20486705	77C4-77C6	FBti0228169	97636

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAATTCAAGGTGAATACTTCCAC	CAGCTCGCACTCAAGTTCATTC

Images

Construct Design Sequences

sgRNA sequence	AATCGAAAGCTTTCAAACAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAATTTATGTAGCAGTCAAAGATAATATTTTAATATTGAACCAATGCTAAAATCGTTGCCGGCATGGGAAAGCGTGCCAAGCACGTTTCGCAAAAGACAAAGACCAGGCCCAAAAAAAAAATCGCAGAAACTGAACAAGACATGGCCAAATTCGGCGAGTTACAGGCGATTCCAAACACCAGAAATCGAAAGCTTTCAAA	CAGTGGTCGATGTTGTAGGTGTGGACAGTTGAGTGGTCTGATTTTGGGCTTATATAATGGAGCAGACCGACCGACAGACAGACAGACAGACAGACGATCAGCGGAGTGTTTAAATAGCTGCCCACCAAAATTGTTGTAGAGGAGACATTGCCAATTGGCATGGAAAGCATGACGATGATTATGCTTTTCCATTCGATTCT

 

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