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CRIMIC CR02839

Gene Sms

CG4300

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:12276785..12276786	69A4-69A5	FBti0228172	97639

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACTTTGCGGTGCTGCACGAGAACAA	AGAAACTGGTGAAAGTGCTGGG

Images

Construct Design Sequences

sgRNA sequence	ATCAGCCGAGACACGGAAACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGGTGATTACCTGCCGCATCTTCCAGCACGGCCTGCTGACGCTCAACGTGGAGTACTTCCTGCCCGATGGCAAGGAGCCGAGCATATCCTTCGACGTACGTATTCAGTGCAATTTAGATTCCTTTAAAAAACTCAAAAAACAAGCAAGCAAACACCCGACAGCAGCACCACCACCCACATCTTTCTTACCCTCCCAGTT	TCCGTGTCTCGGCTGATTAGTTGGGGAAAGGGTTTTCCACCCACCGGGTAAAATTGATTTTCCGCGCACTTGTACCCGAGTGTTGTTGCTTCATCATACATACATGTGGGGGTGTGTGCATGGCAAGTGGGCAACCATGTGGGTGGGTTGATTGGTTGTGTGTGTGAGTGTGTTCCTGACCACTCGGCGAGCTCAAATGG

 

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