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CRIMIC CR02847

Gene CG5021

CG5021

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:8967043..8967044	66E5	FBti0228177	97644

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGAAGTCGAAGAAGTGCTTCACC	AACAGGTGGAAGAAGGTGACGTAC

Images

Construct Design Sequences

sgRNA sequence	CTCGGATCGTATTGTCAGAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCTACGCAGGCAGAAACAACAACAACGTCCCAGGCGAAGAAAATATATTAAATCCCCTGAAAAACCACGAAAATCCATGAAAATTGCACCCAACTAGGAATTAGAAGTCACTCCCTACACCAGCATGGCATCCGCTACGGTAAGAAATGTATGTATGCCGCACTAACACCTAACAAGCTGCTTGATTGGGGTGCCATTC	TGACAATACGATCCGAGGTTTTCCACGAAAACTCATATTTTACAACGAGGCAAGCCAAACTAATCCCACATACGTGTTACACAGGTGCCGCTGCTCGACGATGACACGATACCCTTTGGCGAGGAGGACGAGATGCGGGATCCCAGTCGCGCGGGTCAGAAATACACGTATGCAGAACCATAATGTTTGTTGATCAATTC

 

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