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CRIMIC CR02848

Gene Usp32

CG8334

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:19877801..19877802	76D3	FBti0228178	97645

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGTGCTTTGGAAAGAGAGAGT	GGCCAGCAACAAAGTAAGTGATTAG

Images

Construct Design Sequences

sgRNA sequence	CTCTCGTATGATATATCGTCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCATGCCCCACAGCCTATTTTGCTTCAAGGTATGGCTTCTAAACTATATTAACATATACGTATTTAATATCATAGATTTACGCCCCATCTATTTGGGGCCATATCGAATTCTAAAATTCAGAGCTTCAGAACTTTCCTTGCCTAGGATAGCGAGCTTGCTCCTCAGCGGCGAAACTGCTGCTACTCTCGTATGATATATC	GTCGGGTCGTCTTTGATTGCGAATGCGAGATCCGCCAAGAATCCGCCTCCGTCTGCTTCTCGATTGTCACTTTACAAGCGTTTAATCTAAATATATTAAATACACTATGTTAAATGAACAGTTGCCGTTTGCTGCGTGGTTTGTGGGCTTCCCTCTTATTTACTCAAATGTACTAGCATACTCTGTATATTCTCCCTACT

 

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