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CRIMIC CR02851

Gene CG1265

CG1265

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:4255477..4255478	64A12	FBti0228179	97646

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCACTCTCATATACATGAAGCTC	CATGTGGTTCTACTGACGGAACT

Images

Construct Design Sequences

sgRNA sequence	GAAAAGGCGGTGCTCTTAACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCCTTAAGTTCCTGGTGGTGAGTAAGCCTGTATTGATATCACCTAGTGACTGATTAGTCGGGTATTCTTGCCCAGATAACGACTTCGGCGCAGTTCAAACCCGTTCCCCGCTCTCTTTTCCAATTAACTAATTATGTTTCCGGGCAAACGGGTTTGGTTTGATTTGTTATTTGTTTTAGTTTGAAAAGGCGGTGCTCTT	AACTGGGCAGCATCTTCGCTCAAGGTCACCGATGTTTGTTGTATAATATTTGAGCGGCTATTAATACTTTCAGTGCTCGGGCAATATATATAAATCTCGTGAAATTCATCTAAATGTTTGATTTCCCGTCAGCCTTTCTGCACTCCCATTGGAGCCACGAGTAAGGTCCTCCAGCTGCTGGCTATTCTGAGGACCAAGGA

 

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