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CRIMIC CR02852

Gene GlcAT-P

CG6207

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:11180018..11180019	68B1	FBti0228180	97647

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGCACGATGGGAATTACACTCGAA	ATGTGTGTGTGCACTGCACTTGGA

Images

Construct Design Sequences

sgRNA sequence	GAGCCTGGGCAATCCGAGTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGCAACATGCTTAACTTTTTCAGTTTAATAAGGTTCGAGAGCTGTCTTTAAGAACTTTTCGTTAAATAAGGTCGTATTTACTTGCTTAGTTTTTAATTTGTTAACTTTCTTATATGTTATACTTAACCTTTATTTTTTCGAGTGCCTTTGACTAGCGAAAGGCGACCTATATTGCTTAACCAGAGCCTGGGCAATCCGA	GTTGGGGCAATGATGGGCGGATGTGGCTCGCTAGCTTTCGCCTAGTCAAGTCATTGGGCTGTAAATTAATTTGCATAATTGCTCATAAAAAGTAGCTGCTCACGCCGCATTACGCACCACCCAATGGCACCTAACAAGGCACTCAGCCAAGCTGGCACCCCAATCACCCAGCCACCTAGTCATCTAACTACCATCTAGAC

 

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