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CRIMIC CR02854

Gene PVRAP

CG32406

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:6021556..6021557	65A2-65A3	FBti0228181	97648

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTCAATACAAGGATCGTAGCCATA	GGCTATGGCAAGTTGCCTGTT

Images

Construct Design Sequences

sgRNA sequence	GATATGCCTGCCGTAACATGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACCCCCACACATTCCGCATATGTTCGAGGTCGGAAAACAAAGAAACGCAAGAGAAAAAATAAAAGAAAAGGGTTTGTGCTTTCGCTTTTGGTATTTTTCATATTCCTGTGCAGCGTGCGCTTCCCTTGTTTTGTCAATAAATCAATTTCGGCTATAAACATTTTTCGCCGGCAACAGCAAATAACACGCATACGCCGCAT	GTTACGGCAGGCATATCCTTTTGTTTTGCATAGTTCTTTATTTTTTGAGCCCCCTTTTTTGTGGTTCGACATGCTGCTGCCTGTCAATAACATCATTCTGTTGCAGGGTGCGGCCCGATAAGCAAATTAATTTTTGCTTCCCCCATGGGCGGGACAGAAATGTGGTGTGCGGGCTGGGGAGGGGTCGGGGGCGTTTGCAG

 

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