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CRIMIC CR02857

Gene CG32485

CG32485

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:3050798..3050799	63A5	FBti0228183	97650

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGGACTTCAAGGATCTCAAGGAG	TGTCTCACGCCACTTGTTGGTCTT

Images

Construct Design Sequences

sgRNA sequence	GCTCAAAGGGGCTAATTAAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGAAATTGATTGTGGAGGCGGATCCAAAGCAGTATCACAATGACTTTTCGCTGCGTCGCTATCTGCGCGCCTTCAAGACCACAGATGACGCATTTCAGGTGAGCCGTTCGCAAATAAGGCCCAATAATAACCATTATTTGAAAATAGGAACCTCAGCTCTGAATACCCTTTCTCTAGGTCTCGAACTAATTTCCCCCTT	AATTAGCCCCTTTGAGCTCTGATTAAGTAATAAAATGCCAAGTTGAATGCCTTTTATGGATATAAACCAATGGATGCTTATGATATCATCATCCATATCTTGGACCATATAAAAATTATATGAAATAATGAATACCTTAAAGGGATTTCAGATACTTTTTAGACCTTATGCTGAATAGTAGTACGAACAATGTTTTCCCT

 

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