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CRIMIC CR02858

Gene EMC10

CG32441

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:21575017..21575018	78D7	FBti0228184	97651

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGGCTGCAAACTCGGAAAGTTT	AACGGTAGAACTCGTTTCCAAGGG

Images

Construct Design Sequences

sgRNA sequence	GGAGTGGTTACAGGCGAATGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTCCGCGGGAATGTGACGATTCCCAGTCTCAACTCTGGTCTGGCCAATGTGGAGCAACCAGATCTGAGCACTGCCGATCTGGACTTGCTGAAGGTAACAGTGGTTCGGGCACATGGGCACGCCGGTAGGAAAATGTTCACCATTTTTCACCATCCAAGAGTGGTGTACTTAGTGCATCTTATGGAGTGGTTACAGGCGA	ATGAGGTGGCTAGATATTCGCTGAGTATTGAATATACTGCAGCATTAACCATTGTATTCTATAAGTGTAGAAATGCATTCAATTATATAGCCTGTTTGTTTCCATTTAGTTTTTAAAAAGATGCCTTGTCAGTTAAGTTTAAGCCTTCCTGTTGAACGTAATCTTAAAATAAGTATGCACTTAGTAATAACCCCTTTAAC

 

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