| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_PM37_p0 | 3L:20149614..20198317 | 76F2-76F3 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | CGCCGTAATCAGCAAAAGTTGTTC | GACGAGTCCAAGCAGAATGACA |
 |
| sgRNA sequence | TCTGGAGGCCGCGTGGGAATGGG |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|