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CRIMIC CR02868

Gene SLC22A

CG7442

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:21936739..21936740	79A4	FBti0228191	97656

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACCAGTGTGAAGGTCCAAAGGTA	CGAAGTTGGAGGTCTGAGCTCAAA

Images

Construct Design Sequences

sgRNA sequence	TGCCCCAAGCCTGGCATCTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAATCGAGGAATTTTACCACCCATAGGGTAAACAAATTTAGTTTTCGCGGTCACATCTGTTACCCTAGTCTTTGGACAGTTTATTATGTGCCCTATGATAATGTCGGAATCTGTAGCCTAAGCTTTCTTGCTAAAACATTCTTTATCAGACAATTGGACACGTAGATGGGAGAACAGTGACTGGCAGATAAGTGCCACAG	ATGCCAGGCTTGGGGCACAGCAATTGCAACATGAAAACACAAATTGAGATTAAGATGTTCCTAGTCAAATTGGGTTACAATTTAGGTAAATATTCAGTGGGACTGATAACTTTGACTGAATCCAATTTCCATTAATTTTTCACAAGGTTGATCTATGCTCGGCATAAATCAGTTACGGTAAGAGCATTCAAAGAACTAGG

 

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