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CRIMIC CR02870

Gene Cpr67B

CG3672

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:9405209..9405210	67B4	FBti0228193	97658

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAGCATCTCTGAATAGATCCATCCG	CATGTAGACGAACTCTCCCTTCTC

Images

Construct Design Sequences

sgRNA sequence	TGGGTGGTTAGCAGACTTTATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGGTGACTCTATAAATGTCAAATGTGTCCGGATCGTAAAGTGCAAATACGAAAGTTATACAATGTGCAAAAATTAAGCAAACATCGCGTTACATGTGAGATGAGACTTTAATCACTGATGATGATGATGGTGATATGACATCATACCCCTTCTTTTTGTGATCTCTCGAGAATTGTGTAGCGTGTTAAATTGGCCATAA	AGTCTGCTAACCACCCAAGTCACACTTTAATGGTTTGTCTTAATATTTTGCTAATTATTTTTACGCTTAATTCATTTCAGGCCACACTAGCCAGCGGTGAGAACATCTTCAAGATCAACATAACTCCCGAGGAGGCGCAGCAATTCCTTAACAGCGCCCAACTGCGTGGCATTGGCGACATCGAGTATGCCCCGAAAACC

 

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