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CRIMIC CR02876

Gene dmt

CG8374

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:9713721..9713722	85E5	FBti0228195	97660

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTTGTGCTCCACTATGATTGCGC	AATCCAAGCTCTTGTCGTGCGAGT

Images

Construct Design Sequences

sgRNA sequence	AAGAAAGACTTAAATCTTTGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAATCGATGGGATTGTTTAGGGTTGACCAGGACGAGCCGGAGCAATCATTTGCCGATAAGGTAGGATTTATGTAATGGGTTCGTCCAAGCGTTGGGAAAGCGTCGACACTGTAAACAGTGAGTGTAGACACTGACCTCATAACGCTAGATTAACTTAAAACTGAGACGTTAACGTTTTAGTTGAGCAAACAGGGCCGCAA	AGATTTAAGTCTTTCTTTGATCGGTAGTAAGCCGCTCACACAAATAAAAATTTTAGTCGTAAGTCTGTAGTTTTGTATACAACATTTGAAATCTTTTATTTTGGCAAACGGCGCCTAATTGTTTTAAAAATTCAACCTGTTCTAGCAACCCAGACGAACCTATGTGAAGGAGCGTCCGCAACGTAAGCGAAAGAAGCGAG

 

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