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CRIMIC CR02878

Gene CG31109

CG31109

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:25054290..25054291	96B19	FBti0228197	97661

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGAATCGGAATCAATCAAGATGGG	AACTTAAACTTGCGCTCCTCCC

Images

Construct Design Sequences

sgRNA sequence	ACACAAACATCCTTGACGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATAGAAAAGGCGTGCATATCGGGATTTCTATGCCGACTGTGTTCAGAGATGCACCGCACAGTAATACATATTTATAGTGATCATGGCCAGCGTTTGTGTCTTGTTGAGAAAATCAACGGTTACCTACCAATTACAGTGAGTATTGCCAACTAATGGACGCTGCTGAAAAGGCGGAGCTACACACAAATCACGTTCCCATC	GTCAAGGATGTTTGTGTGTACCCAATGTCAGCCATTCCAATAGTTTTTCCCAACTATCAGCATCTTGTTAAATAATTTATTGATTTATCACCTACTGCTCAATTGCACCAGATATCTCCGACTGATCCATTGCCAAAAACCATATGCAAATCATGTCTGCATCGCGTGGAGCAGCATTACTCGCTCCTGATGCGTCTGAC

 

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