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CRIMIC CR02880

Gene CG1142

CG1142

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:7473034..7473035	84D8	FBti0228199	97663

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCAACAGAGTGAGTGTGAAGCG	TTCGTTTCGCATCTCGTCGGTGAT

Images

Construct Design Sequences

sgRNA sequence	AGAGACTAAACTATCTGTCTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAACAGCCCTTTTGCCCATGATGAAATATTCGACATCGGAGTTTGCGCATTGGCGACAAGACCTAAGGCTCAGACTCTAGCAAATCTAGTTTCATCCGTTGTAATCTTTGCTGCTGCACTCCAGAATTTGCAGTGGCCGTAAGGCCAGCGGGACACCAACAGCCAGGGATCAAGACTATTATCTGTCATTGAGTCCTAGA	CAGATAGTTTAGTCTCTGGTTTACAGAACACCCAAATGGAAATGAAACACGCGCTTTTGTCGACCCAGGAGCCAGTTAGGTCTAAGCTGTTTGTCATGGTGACGACGTCTTTAGTAATCTGAGCGCAACATATTGGAAATTTCAGACCCTACCTTGCTTTTATTTTTAATCGCCTTCCTTTTTTCAGGCTGAACGCTCAA

 

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