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CRIMIC CR02884

Gene Syx18

CG13626

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:24624270..24624271	96A12-96A13	FBti0228202	97666

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGACATTGCCCAACTGCAGGACA	AGAACGACATGACCAGCAGGAAGA

Images

Construct Design Sequences

sgRNA sequence	CACATCCTTCGCGATTACAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATGGCATGATAACACAAGTCCCAAGGAATCCTTGCTGTTCTTAACAACGATATCTTTAGTTTAATTTTGGTGAGAACATATTGCTCCATATTGCCAAGAATACTTAGTGGGTTTAAGTGGCTGCCCATATGTTTGTCGACTTAATCCTTCAATTAAAAATATTAATTGGCTTCGCTAAAATCCACATCCTTCGCGATTA	CAATGGATGTATAAGCCGGGCTCTTATTAGCATGCAATTAATTATTAACTAGATCCAGATAGATCCACGTCCTAATCTCATTTAATCCTCTTAAATTTCCCTTTCCCTTTTGTAGGTCGCCATGCAACAGCACAATATCGACAGGATAGTTAGTGCCGTCGTGGGCACCACGGAAAACGTCAAGGACGCCAACGAGCAAA

 

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