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CRIMIC CR02892

Gene CG4733

CG4733

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:19895602..19895603	92B4-92B6	FBti0228206	97669

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTTCTTGCCAAGCGTCTGATCA	CATTGAATTCCATATCGTGGCAGC

Images

Construct Design Sequences

sgRNA sequence	CTTCTCGACGTGGTCGAGTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAATTGAGTTTTAAGCAGGCGGCAGACAGAAGTTCGATCTGCTGGTGCTTTTTCGTCTCTTTGCTCTTTGTCTACAAATTGGGCAACAGTGCGTATGCGTAATATTTGACTATAAACGATGTGTAACCGGGCGTATACGTAATATGCGCACATGCCAATGAATTGTTTTCTCGATCGCAGAGGCTTCTCGACGTGGTCGA	GTGAGGAGATTAATATTTATGTCGCCGTTGACAGTTTTAAAATCATGAATGGCCTGGGCATGAATTATATTCGATGAATAAATAGTCCTCACACGAATAAAAGCCAAATAAAGTCATTTCATGTTGATGTGAAAAACATAGAAAAACAATAGATTTAGTTGAACTGAATTTAAAAGTTAAATCTGTTTTAATCAGCATAG

 

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