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CRIMIC CR02894

Gene Arl6IP1

CG10326

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:16644453..16644454	89D6	FBti0228208	97671

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAACTACGCATTCCCCATGGTGT	AATCTTCGAGGCCCTTTGGGTGAT

Images

Construct Design Sequences

sgRNA sequence	GAAGATCTACTGCACATACTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTTGGCGGAGTCAACTGGGATGGCGACCAGGAGGCCAAGTTCGAGGACGTGTGCGGCCAGGTGTGCGCCGTCAAGGGCAGCCTGGTGGTCTGGTACGAGTACCTCTTCAACGAGCGGAAGTCCACAGTGGTGAGTAGATGCGGTAGAGACCGTGTGTGTATATGTATATGAAAACAGAGAAGCAGTTTCAGGGCCTAGT	ATGTGCAGTAGATCTTCTTCTGCAAGCTCAAGTGTAAATAAAAACTTCGGGTTCTATTATTATTTCATCTCACATTTATTCGTTTTGATTCCAGTTTGTAATCGTCATGAGTTTGGGTCTTTTGGCTATGGCCTGGATTGGAGCCATCATTAACAATCTACTGCTTATGTACTTGGCCACCCTACTCATCCTCATGTGGC

 

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