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CRIMIC CR02896

Gene aralar1

CG2139

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:30450130..30450131	99F4-99F5	FBti0228210	97673

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTACGCTCCACTCGCAGTTAGTT	GTACTTCAGGAACACCTCTCGCAA

Images

Construct Design Sequences

sgRNA sequence	GAGTGCGACTGAGACAGTGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATGAGCTAATTAGACGCGGGAATTGCTCGCGGAAACAACACTGAACCAGAAGCAGTCAAAGCTAAAAAACAGGAATGCCGTTGACCAAGAGTTTGCCAAATGTAAGTTTCAGCTGCGATTGCCAAGCGACTGACACGTGTTGCTTTTGCAATTGACTGTCAGGCGGGAGAGCGCAGAAATGAGAGTGCGACTGAGACAG	TGGCGGGTAGCGAAGGGTTGTTTGTGAACTACCCATAAAGATAAAAGTATAAGTAAATACGTTCATATATACAGCAAAAAGATATTCAAAATAATCAAGTAGAGGAGAAGAAACCCCAATGAAGCAACCCTTTACCACAACTAATTATTTACTTTGCAATTCTTCTTGCAGTCTCCGTCGCTTTTGAAGCGCGCCGGCAC

 

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