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CRIMIC CR02898

Gene side-IV

CG14372

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:13458026..13458027	87E12	FBti0228211	97674

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCTATGACGTGGAGTGCAAGAGT	ATGAGTCGCCATTTGTCCTCGATG

Images

Construct Design Sequences

sgRNA sequence	GCCGGGTTTCGCCGTGGCCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGCCGGCGCTCATCACCTGGTGGAAAGGCAGCAAGCAGCTAAAGAAGCTAACCAAGAATGTAAGCCCCGACTCGTAAATGACTCGTGAAGGCGCTCAGTCCCAAACTCATTAATTAAACACAAATTAATATTGTGTAGTGTGTGCGTCGCTTATAATTGCCACAAATTTCCTTTCCATTTTCGCCGGGTTTCGCCGTGG	CCTTGGCACGATTTTCTTTACTTTTCTCACTTTCCGTTCCGTTGCGTTCCGTTCGGTTACTTTCGGTCCGCTTCCGTTTCATTATTCATTTCCTAATGGACTCTGTTTGCGTTTCTTTTTTAGTTTAATGAACCCGATAATCAATCTCTAAGTATACTGACGTTTACGCCCGGACGCGAGGACGATGGCAAATATTTAAC

 

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