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CRIMIC CR02901

Gene CG5214

CG5214

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:11244302..11246862	86D9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTTCGATCGTAACGAGACGGCTG	TGCCAGGTGAGAAGCCTAGGAATA

Images

Construct Design Sequences

sgRNA sequence	GCTGACGGGTCACGTGCAGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAATTGTGGACGGGCTGGGGATTGCCGGGTGGAGGGGGCCCGTCAGGTGTGTGATATATGCTCCGGCGATATATATGTACATATCAATTTTTATCCCATATTCACTGCCCGCCTGGCGCTCGCCGATTATGTAATTGTATGGGTGTGTGTGCGTGCGCCGCTGAAAAATGGGAAAAACTTTTGTTTGGGACGCCCGCT	GCACGTGACCCGTCAGCCGACGTTGCCTCCTGGTTGTGCAATCACTAATATTTGCTTCTTTCCGCCAGGTAAAGCGCTGCATCCGCCAATATTCTCGGTTAGTTGCCTGCGCCGCCCAGCAGCAGCAACTGCTTCGCCAGGATGGCTCGAATCGCTGCCAGGAGGCCACCAGGTAAGTCCTTCACTATCCACAAGAGTCC

 

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