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CRIMIC CR02906

Gene Pcd

CG1963

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:29723163..29724226	99B9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCAGTTCTGTATTCGCCAGCAG	TCCTTCAGCACGAACTGCTTGAAG

Images

Construct Design Sequences

sgRNA sequence	TCAATCGACCACCCAAGGACAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAACTGGTGGCAGTGCGAATCTCCGGAGAATCCACAACACCCCAACCACAGAAGCAACAGCAACCGCAATCACAGCGAAAAAGCGCAAAATGGTGGTAAGTACGAGCCGAAAGTGAGTTTGAGATCAAGGTGAACGGAGTCTCAGCGAAATCGAGTGGTTCCCTTATCGCCTCCAACTGAAATCAATCGACCACCCAAG	GACAGGTTTGGACTTTCGGAAAGTGCTTTATGCCCTAACAAATAGAATCACTGTGATTTTGGCCAGAAATGGAGGTTTTTCGCTCACAAATTATTTTGTATATATATCGTATGAATCAATAACACTCACATACTTGTTGCTCGCTGCCCTTTAGGTACGACTAAACGAACAGGAACGCGCCGAGAAGCTGCAGCCCCTCC

 

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