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CRIMIC CR02907

Gene SP1029

CG11956

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:29246041..29246042	99A6-99A5	FBti0228216	97677

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAGACTGATCGAGTATCTGCAGC	AACCGGATTCTTCTCGTCGGGATT

Images

Construct Design Sequences

sgRNA sequence	TCTATACACCTAACTTGTCATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCCTTCGAGAATCTGAAGCGTGTGGGCCGCATTGTCCGCCAGACACCCGATTTCGAGTTCTTCAAGGTGGGTTCTGGTCTCTTAAAGTATCCTAATGCTCTTCTACCACGTGCTCCCCGTATAATCTCATTCTCATTTGTATTTCGAAACAATCCTTTCAAAATTGCTCACACCTAACTTCATCTATACACCTAACTTG	TCATGGGCAACCCACCGACAGTTGTTCTTTGTCGTAAGTCTGACTAGATAGCACTAGATATTTTATAATTTGACAAATGCCGGGTATATGAACTTTTATTAAAGTCCTTTTAAATGAATTTATTCATCCTCAATGCAGCGCTACATGAAGAAGCTGATTCTGCCGATCTACGAGCACCTGAATGGCATCAACGACACATT

 

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