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CRIMIC CR02908

Gene Ef1gamma

CG11901

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:29220362..29220363	99A1	FBti0228217	97678

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATCGCCGTAACCATGATGACTCC	ATCGTGTTGATGACCTGGCACTAG

Images

Construct Design Sequences

sgRNA sequence	TGGAAATTCGCAAATTCACATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGCAGCCCTGTTTCCGTCCCTTCTCTTCTTCGTTTCCGGTGAGTGCGTGTGCTCCGCGTTTTTTCGCTGAATTTGCCACTATATCGTATTTAATACTGTATTTGCAGGCAGACGATTTAAACGGCGAATCCACCATGGTGAAAGGAGTACGTACGCACAAGGGCAGTGAGGATTTTCCAATCCGTGGCTTCGAGCCATGT	GAATTTGCGAATTTCCAGATGCCGGTTAGCTGCCACGATATCACAACTAATCCACCCATATTTGCAGACTCTGTACACTTACCCCGAGAACTTCCGGGCCTACAAGGCGCTCATCGCAGCCCAGTACTCCGGAGCCCAGGTGAAAGTGGCCGACAACTTCAAGTTCGGCGAGACCAACAAGTCGGCTGAGTTCCTCAAGA

 

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