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CRIMIC CR02916

Gene Naa15-16

CG12202

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:19479984..19484643	18C7		98472

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATTCACGGCCAGGCGAAAA	ACAGGATCTGCTTGGCCAGTTT

Images

Construct Design Sequences

sgRNA sequence	AGGCACTCTCCACCTGACTTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCGCAGCCTTGCAGAGATGCCTTCTAGCGATCCCCTGCCGCCCAAGGAGGGCGCGCTCTTCCGCAAGCTACTTGTAAGCCGGTCTAGGTGACCCTACTGTCCCATACCTTCCCCTTCCCTTAATACCACAACTGTGCCGCCCCCTCCCTTTGTTCGTGCCTTTGGCCGGCTTCGGCATCCATGTGCTTTTTAGCCGAAG	TCAGGTGGAGAGTGCCTATTTTTGGCAGAAGCAATCCGGAGGCGATGCCTCCGCTGCTCGAGATGTGTGGCTTCAGCGGGCGATTGCACAGCTCTGCTCAACCGCCGACGTTTGTGGTCGGCCCGCTGGTCTCATAACCTCAAAATTCGCGCTGCGCGGGATGCGAGAAACTTTGACTAAAACCTTTCTTCTCTGTGCTT

 

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