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CRIMIC CR02918

Gene Trf4-1

CG11265

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:8566130..8566131	7F4	FBti0228223	97684

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ATGACTCCGAGGATAGCAAGGA	GTTGCTGTTGATCCTCAAGCTC

Images

Construct Design Sequences

sgRNA sequence	ATGCCCAGGCATCAGGTGGTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGCCAAATGGCCTGGCCAACATGTCCATGCCCATGCCAGTGCATGCTGTCGGAATGCCTGCGTCGAACAGTTGGAGCGGTAATGGCAATGGAAACGGAAACAGCAGCTCATCCACGGGCTCGTCGGTAATGCACACAATCCCCCGCCTTGACACATGATGATTGGTGGTGGCCGGCTGCCTAATGCCCAGGCATCAGGT	GGTCGGCTATGCTCCTATGCTGGCCGCAGTTGAGTCCCGGTTGTATCTGAAACCGAAAGCCCAAACAAAATTTCATCCAAAGACCATGGGAGCAACTGCAGCCAGCATGACTAGAAGCAACAGCAGCAGCAAGCACACATATATAACCTCTTCACCACCAAAACAGCCGGAAATCGCACACATCGCTGCCCAGGAAATGG

 

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