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CRIMIC CR02922

Gene CG12730

CG12730

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:5517982..5517983	5A2	FBti0228224	97685

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGGTATCTCCGGTTGTGGCA	GCAAGCGATACTCGCTCTCTCAAA

Images

Construct Design Sequences

sgRNA sequence	CAGAGAGTTCATCACAGCGAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTACTTCACGGCCTCCGGACTCGTACTCTCCCTGGATATTGGAGCCTATACGGCGGCGGCGGTAAGTGGCGTTCTCGCAGTTAGGATAAGTTCGGAAACCAAAAGTATTACGGCTCATTGTTTCGGCTAATGATTAAGCTAGGAAAGATACTGGTAAACGAAACACTGAGCCTCCAGTTGGAGCCGATTCATTCCCTCG	CTGTGATGAACTCTCTGTAGATCCGTCTAATACACCCTATTCTTCACCTTTTTTTTTACAGTTCTTTGGCCTGACAGCATTTTGCATCAACGGACTGGAGGCCTATGGAAACTATCGGCGCAGTCGCCAGCGGGAGGTGGCCACCCAAACGATATAGACTTGGTTTCAGGTGCACGAACCCAGCTTTTGGTGGCTCTTAC

 

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