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CRIMIC CR02932

Gene RSG7

CG9108

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:16842888..16842889	15B1	FBti0228229	97690

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGATGCACCCAATATCCTAGTG	TCAGCGCATGATCATCGATGGGAA

Images

Construct Design Sequences

sgRNA sequence	CTGACTGGCAATCAATAAGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTATTGAGCCTTACCCATACTTACTTAGCCAAAGTTATCATTTCATTTCAACTTTATACTTTAATGCATATACATATGTATGTGTAAACCTTTGATTGTTACCTTGAACAATGCCTTGCAAATTTGCATTCGCTAGATAATTGCATTTCACTTTCTGTCTTTGGAGTGGTTCATTATCTTTATTATGCACATGCCAGCT	TATTGATTGCCAGTCAGTTGTGCCGCTAATTGGAGCTAATTAAATCGATTCCATTCCCAGATGGAGGCCATCATCGAGAAGATGCAGGCGGAGTCGACGGGCGTGGCGGTGCGCACGGTGAAGGCGTTCATGAGCAAGGTGCCATCGGTGTTCACGGGTGCGGATCTGGTCGCCTGGATTCTCAAGAACTTCGACGTGGA

 

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