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CRIMIC CR02938

Gene mbt

CG18582

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:16610685..16614856	14F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTGCAAGCAATGCTTGAAGGCT	GGAAGATAGCATAAACAGGTTGGG

Images

Construct Design Sequences

sgRNA sequence	GCGATTTCACCTGGCACTTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACCATTCCCTTGGCCAAACTGGGCAGCAGAGAACGGAGTACTCGCCATCATGCGATTCTTTGTGCCTTTGTACCTTGCCATTTGCGGCGCTTTCTTCGCCCTCCGCTTTCGCGTGGCTTTGGTTTCAATTTCTAAATGTTGTCACCAACAAAAGTCGTCGGCCGCACAGTGGGCCATCGAGTGGCGTAGCACGCCCAAA	GTGCCAGGTGAAATCGCACGTGCATGCCCTACCTCAAAGCGAAAAGTGTGCATAGAGCGATTTTCGAATGGATAGTTAATTGCATCTTATGATGAAGGAAGTTTAAAGTTATAGTTCATTACTCATTGATAGATAAGCCAATTTGCTATGATTTTGCCTGAGATACAACTTGAGATATTAAAACTATCAATATTAGAATA

 

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