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CRIMIC CR02942

Gene Hr4

CG43934

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:1941940..2007956	2C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTACGTGGTTCGATTTGCAAAACC	CTCGATCTTGATCTTGGGCAGGTT

Images

Construct Design Sequences

sgRNA sequence	GGTGATGGCTTTGTCGGAAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTACCTTCTCGTTCGTTTTGTAAATCCAGCAGAAGTGGAACTGGAGAGCATCCAGGAGCGGTACGTCCAGGTGCTGCGCTCCTACCTGCAAAACTCCTCGCCGCAGAATCCGCAGGCGAGGCTCAGTGAACTGCTCTCCCACATACCAGAGGTAAGTGCCCACTAACTATGTATATGTACTTCTTGGCGATTCCCATTT	CCGACAAAGCCATCACCATCTGTCCTGTGATTCATCGCTCTCCCCTATCCCCTTGCAGATCCAGGCTGCGGCTAGCCTGCTGCTCGAGAGCAAGATGTTCTATGTGCCCTTCGTGCTCAACTCGGCGAGCATAAGGTAGCAGATGCTTGAGCACGGACTGCTGCCCACCGCCAACCATCAGTCGGCGCCACCACCTGGTC

 

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