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CRIMIC CR02943

Gene Clic

CG10997

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:13812971..13822307	12C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GAGTCAAAGAAAGATTACGAACCCTTTGG	GGGATGGGTTTCGAAAGTGTTAGG

Images

Construct Design Sequences

sgRNA sequence	GGTGCGGTCTCCATTTGCGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTATAGTAGAGGCCGCAATCTATTTCTTTCCCCGGAATTGTAAGTCGAGCATGAGTCCACTTAATTTTGTTTCTTTTGTTGGTTCCCACTGTTTGCCGAAGTAATTGTAATTGTTTTCGGAACACTTTGGAGCCCTGCTGTTGTTGATTCTATTGGCAAACTCGACTCGACTCAACTTGGCCACTCATCCAGCCCACG	CAAATGGAGACCGCACCTCGAGTCGTCTGCCCATGAGTAATTTCCCTCACCTTTGCATTGCCTAAACCCAAACAGTACAATATACCATGTGTTATACCATTCGATTCCCGAAAGAAAAAGAAAAAAAAAACCCCACTCGACGCTAAACAACTGACATGAGCAATCGTGTACTTCGAATAGGGTTTCGTAACCAGTTTTGT

 

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