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CRIMIC CR02953

Gene nonC

CG32743

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:6833449..6833450	6D3-6D4	FBti0228241	97702

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCAACTGGGATACGTGGTAA	CATCTGTCGTACAGCGCACAGATA

Images

Construct Design Sequences

sgRNA sequence	TTTGCTAAGGATGACATGGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGAGACCAACAGTACAACCAGTACTATTAGGATTGACATGGTTATCCACTCTTGGTTCTTTTAGAATTAGAAATCAGGAGAATATATAGTATATATAGGTACAACTCTCTGATCTAATCCCATATTTGAATTATCTTTAAGATGAGATATTAAAGATTTCATGGATCAGAACAGACCAGGAGTTTGCTAAGGATGACAT	GGCTGGCCTACTGAATAAATGGCGTAAATCTGGTGTCCATTATATATATTTATATATATATATATATATATATATATATATATGGACAGACATTGTGGCTTATCACGCAAATTGCGGCCCAATCTACAATTGTAAACAGCGATTAGCGTCGAGGTCGTATATGTAGAACATGGTTATGGTTTCGGGCTTGTTTCTGGTTC

 

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