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CRIMIC CR02961

Gene Tep5Psi

CG12567

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:19558969..19558970	37F1	FBti0228244	97705

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGGCAAGCAATGTGGGGTAAGT	ACCACTGGAGTATCGTGTTGGAAG

Images

Construct Design Sequences

sgRNA sequence	CGATTAGGGATTATGGTTAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCATATATATATTCTAAAGTTCTGACGACAAATCATTTTAACACTACAGTACTATTTAACTATCTACTCATTTCACAAAGATACTCAGAGTCAAGAAAAGCTTAAGAATATTTTTACTTATAGTATAAGGGATTAAATTAATATAATATTCTGCACATCTTCATTCATTTTTAGACTGTATAGTGTAGTGGCGCCCTTA	ACCATAATCCCTAATCGGGACTTTAAAGTAACCGTTGCCGTTTATCACACCAAAGAACCAGTCATCCTGAAACTTGCCATCTCATAATGCTTCCCAAACCGTGGATCTACCCACGGACCGAGAGATTAAAGAGGACACCTTTAAAGTGCCTGACATTTCCTCGGGTGACTGTGCGCTGACTGCTGAAGGAGTGTCGGGGC

 

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