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CRIMIC CR02962

Gene CG31676

CG31676

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:20841111..20841112	38F2	FBti0228245	97706

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGAGTATCGTTAGCTAAGGC	ACGCTGTGCAGACAGATGGCAT

Images

Construct Design Sequences

sgRNA sequence	GCAGTCGGAGTCTTGGGGCACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTGCTTGTGCAGTGATATACCTGGAATCAACCGAGAAACGCATAAAAAGAAGGAATTATTGTATCATGGCCTACGGTTCAACCGGCTGCCTCTTGCTGGGCCTTTTCTCCTTGCTGATGGTTTTCAATACAGGTGTGTTATTAAGCCACTGAATCCGCCTCGAATGGGGGAGCTAATTAAGCGCAGTCGGAGTCTTGGG	GCACGGAGTTGCTTAAGTGCGTGGGCGTGATTTCCCATTTAAATGTGCAACAGAACGCCAAGCTGGAGTTCACTGAACCACATCTAAAGGGTTCGCTTGTGCGGAAAATGGGGGAAAACTGAGTCAGATTAAAGTATTTGGCAGCGGTTGAAAGCAGGGCGAATTTAAAGCAAAGCTTTTAAAGGTGCAGAAATATAAAT

 

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