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CRIMIC CR02963

Gene Fs(2)Ket

CG2637

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:20735653..20735654	38E5	FBti0228246	97707

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAAGACATGACTTCCGATATCG	GGGAATGTGAAGGCAAGATAGG

Images

Construct Design Sequences

sgRNA sequence	GTTCGTACATCCTTAATTGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTGTGTGTATAGATCCGAAAAATATGGATTTTTCTTCGATTGGCGGGAGGGACGCGCGTTATATGTTGCGCAGGGTTGCAATAGGCGCTCGCATATGTTTGGTTGCTAGGGGCCTCCGCACACATGTGCATCCACGTACATACTCCAAGGGAAACTTGGCGGCGCGTCTGTATGTATTCGCAATGCTTGCGCTCCTACA	ATTAAGGATGTACGAACCGCCTGCGACATAACCTCACTAACTCTAGGAATCGATGAGGCGTCGCTTCGGAGTCACCACCGTATGCTTGGCGGCCAGTTCTTCCGGTTGCTGATAAGGAATGCCTGCAAACTTGCCCCTTGCCTGAAGATTTTATGCTCCACCGCTACAATTCATTACTAGACCTTACTGCTTAGCTCTTC

 

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