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CRIMIC CR02969

Gene Ric

CG8418

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:16109501..16109502	52E5	FBti0228251	97712

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAAGCGATCCAGCAAGCTGA	TAGGAGTCCTCTGCAGCAGCAAA

Images

Construct Design Sequences

sgRNA sequence	TTCGTGGGTGTCGGTGGTACGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGCCGCAGAATATGCGCGGCGGTCTGCGGGTCTATAAGATTGTCATCCTGGGCGACGGTGGCGTTGGCAAGTCAGGTGAGCTATTCTAGCCATGGATTAGACGAAAGAAAATGCCGAGTTATCACTGCGCACTTTGATTGGGGCTGCGAAAAATCAATAAATTATCTCAATTTCTTTTACGCTTTGTTTTGACCCCCGTA	CCACCGACACCCACGAAACTCCACCAACCACCACTCCCAACGCCCTCTGAAAACGACGACGAAACCCGAACAGCTGTCACCCTTCAGTTCGTGAGCCACAGTTTCCTGGACTACCACGACCCCACAATTGGTGAGTAAGGCGCAGGGTGCGCAAGGCGTCTAAGGCACAAGCCCCCACAACCCCACTAAATGTATACCGG

 

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