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CRIMIC CR02974

Gene CG1299

CG1299

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:4149428..4149429	64A8	FBti0228254	97715

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAATGAGTTTAACCCAATAATACCGAT	GCTCTTTCTAATGTGATTCAGAGC

Images

Construct Design Sequences

sgRNA sequence	CAAAATCCGACCTTGGTGACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGCAAAGACCTCTAACCTGTGAAAACTTGACGACATCTTCAAGTTCAATGGCGAGTTCGTTGTTTAAGTCTTTCGATTTGACTGCGGTCCGAATGCGGCGATTTGTATTAACTGCAAAACAAACAATCATCCCCGCTTATTCACAACGAAATTTTTGGCTCATTGTATTCAAGTTCACTACCAATTCACAAAACCAGTC	ACCAAGGTCGGATTTTGACTCGGACACCGACTTAAACAGGTGTTTATTACCTGCGGGGCGTTCCCTTAGAGCACGTATCGCATTGTGACCGGTACTCGTAAAGTTCGATTGAGATATAGACTTCTAAGATTCCGTTGACCAGCAAACGACGATCTTTACGACTATCTAAGATTGTTAAGATTATGAGAAAGTCTTATGCA

 

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