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CRIMIC CR02978

Gene Syn1

CG7152

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:21757096..21757097	78E6-78F1	FBti0228256	97717

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGATCTTGGGTTGAACTTCACC	CGAGATGGAACTGAAGTTACGC

Images

Construct Design Sequences

sgRNA sequence	TGGGTGCCTCCTATTTATGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGCGGAAGCCAGCAGCCGGATAGGGTGTGGATGAGTACTCCACCTGGCGTTGACTCTTCGCCCGACGCTGTGGCATGGCGGAAACGGCGGAGAGGGCCAAGAGGCACACGGCCAGAATCAGGATGGGTTGGCTCTTCATGTTGATTGGTGGACGAGTGCTAACTGTTTGTGCCCGGCTGCTGTGGGTGCCTCCTATTTA	TGCGGGGAGTGCCACCGATGCTCCCATCAGAATTCAGTAACGGTCGCAAGGTCGCCATCGCCGGGGGTTATGATAACTCACTCGCTCCCAGATGCATAGTACGAATGCGAATTTGTGGAACAACTGACAAACTGGCGATATTCGCGCATTTAAAAACGTATCGTGGCCCATCAAAGCAGCCATCAAGCTCAATGTTAATG

 

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