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CRIMIC CR02979

Gene CG11370

CG11370

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:22712434..22712435	79F6-79F6	FBti0228257	97718

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTTTAGCTCCTGCTGTGATCAGTG	GAGTACGTACCCTTCAGGTAGGTT

Images

Construct Design Sequences

sgRNA sequence	TGGTAGGTGAAGTGATTAGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGACCCTCGCCCCCGCTATAAGGATTTAAATCACTAGTTCTGCCCCCTCCAAAACTAAGTTCAGTCCGACATGAAGTTCTGTGCTGCCGTGGCTCTGCTACTGATCGCCGGCATTGTGGCCAGCGGCGATGCCCTGCCCGCCAGGAAAAGTGAGTTCGCCGCCCAGAGGATTACACATTCCGCTAGCACAATCACCATCT	AATCACTTCACCTACCACCCTCCATCCCTTCGCCACCATACTGAACACCGGCAATACCTCGTTCCAGGAATGGTCTACCTCCAGCAGCCCGCAGCAGCAGAGTGGTACGGATCCGCACCCCATCAGCGCTTCATGTATATGCAGTATGTCCAGCCCGGCCGCACCCACGCCCGTTCCACCCAGGCAGCCAGTGCCCTCGT

 

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